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VPS33A Polyclonal antibody

VPS33A Polyclonal Antibody for WB, IP, ELISA
Cat No. 16896-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (1)

Vacuolar protein sorting-associated protein 33A, hVPS33A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman brain tissue, HepG2 cells, human cerebellum tissue, NIH/3T3 cells
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16896-1-AP targets VPS33A in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 VPS33A fusion protein Ag10422 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 33 homolog A (S. cerevisiae)
别名 Vacuolar protein sorting-associated protein 33A, hVPS33A
计算分子量 596 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC016617
基因名称 VPS33A
Gene ID (NCBI) 65082
RRIDAB_2214916
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96AX1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS33A is a Sec1/Munc18 family member and is a component of the homotypic fusion and protein sorting (HOPS)-tethering complex. The mammalian HOPS complex comprises six subunits (VPS11, VPS16, VPS18, VPS33A, VPS39, and VPS41) (PMID: 23901104). This complex is required for late endosome-lysosome and autophagosome-lysosome fusion (PMID: 24554770). VPS33A plays a role in vesicular transport to the lysosomal compartment.

实验方案

Product Specific Protocols
WB protocol for VPS33A antibody 16896-1-APDownload protocol
IP protocol for VPS33A antibody 16896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Authors - Shen Zhang
mouseWB

J Hazard Mater

VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes

Authors - Tao Wang
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
humanWB

Autophagy

Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.

Authors - Qiuhong Shen
humanIF

Cell Rep

Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes

Authors - Ariane Schleinitz
humanWB

J Cell Biol

The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.

Authors - Rituraj Marwaha
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