验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse brain tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human colon tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20778-1-AP targets WDR24 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | WDR24 fusion protein Ag14774 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 24 |
| 别名 | WD repeat-containing protein 24, JFP7, GATOR2 complex protein WDR24, EC:2.3.2.27, C16orf21 |
| 计算分子量 | 920 aa, 102 kDa |
| 观测分子量 | 102 kDa, 88 kDa |
| GenBank蛋白编号 | BC008025 |
| 基因名称 | WDR24 |
| Gene ID (NCBI) | 84219 |
| RRID | AB_10696183 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96S15 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR24 antibody 20778-1-AP | Download protocol |
| IHC protocol for WDR24 antibody 20778-1-AP | Download protocol |
| IF protocol for WDR24 antibody 20778-1-AP | Download protocol |
| IP protocol for WDR24 antibody 20778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine. | ||
Nat Commun Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation |
