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验证数据展示

  • WB analysis of HEK-293 using 20778-1-AP

    WB analysis of HEK-293 using 20778-1-AP

    WB result of WDR24 antibody (20778-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells.

  • WB analysis using 20778-1-AP

    WB analysis using 20778-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 20778-1-AP

    IP experiment of HeLa using 20778-1-AP

    IP result of anti-WDR24 (IP:20778-1-AP, 4ug; Detection:20778-1-AP 1:3000) with HeLa cells lysate 1560 ug.

  • IHC staining of human colon cancer using 20778-1-AP

    IHC staining of human colon cancer using 20778-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 20778-1-AP

    IHC staining of human colon cancer using 20778-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 20778-1-AP

    IF Staining of HeLa using 20778-1-AP

    Immunofluorescent analysis of Hela cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Featured Product
  • KD/KO Validated

WDR24 Polyclonal antibody

WDR24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20778-1-AP
Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Synonyms

WD repeat-containing protein 24, JFP7, GATOR2 complex protein WDR24, EC:2.3.2.27, C16orf21

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20778-1-AP targets WDR24 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen WDR24 fusion protein Ag14774 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 24
Synonyms WD repeat-containing protein 24, JFP7, GATOR2 complex protein WDR24, EC:2.3.2.27, C16orf21
Calculated Molecular Weight 920 aa, 102 kDa
Observed Molecular Weight 102 kDa, 88 kDa
GenBank Accession NumberBC008025
Gene Symbol WDR24
Gene ID (NCBI) 84219
RRIDAB_10696183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96S15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

实验方案

Product Specific Protocols
WB protocol for WDR24 antibody 20778-1-APDownload protocol
IHC protocol for WDR24 antibody 20778-1-APDownload protocol
IF protocol for WDR24 antibody 20778-1-APDownload protocol
IP protocol for WDR24 antibody 20778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB,IP

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
View Article
humanIP,WB

Nature

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Authors - Lingyun Long
View Article
humanWB,IP

Nature

Structure of the nutrient-sensing hub GATOR2.

Authors - Max L Valenstein
View Article
mouseWB,CoIP

Science

Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.

Authors - Andrew L Cangelosi
View Article
humanWB

Nature

SZT2 dictates GATOR control of mTORC1 signalling.

Authors - Min Peng
  • KO Validated
View Article
humanWB

Nat Commun

Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation

Authors - Xing Chen
View Article