验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, HEK-293 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11558-1-AP targets WFS1 in WB, IHC, IF-P, IP, coIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, coIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | WFS1 fusion protein Ag2114 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Wolfram syndrome 1 (wolframin) |
| 别名 | WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN |
| 计算分子量 | 890 aa, 100 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC030130 |
| 基因名称 | WFS1 |
| Gene ID (NCBI) | 7466 |
| RRID | AB_2216046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O76024 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WFS1 antibody 11558-1-AP | Download protocol |
| IHC protocol for WFS1 antibody 11558-1-AP | Download protocol |
| IF protocol for WFS1 antibody 11558-1-AP | Download protocol |
| IP protocol for WFS1 antibody 11558-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell A Hippocampus-Accumbens Tripartite Neuronal Motif Guides Appetitive Memory in Space. | ||
