验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells, COLO 320 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue, rat testis tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy XRCC4 Ready-To-Use IHC Kit. XRCC4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15817-1-AP targets XRCC4 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | XRCC4 fusion protein Ag8606 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | X-ray repair complementing defective repair in Chinese hamster cells 4 |
| 别名 | X-ray repair cross-complementing protein 4, Protein XRCC4, C-terminus, hXRCC4, DNA repair protein XRCC4 |
| 计算分子量 | 310 aa, 35 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC005259 |
| 基因名称 | XRCC4 |
| Gene ID (NCBI) | 7518 |
| RRID | AB_2257478 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13426 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XRCC4 antibody 15817-1-AP | Download protocol |
| IHC protocol for XRCC4 antibody 15817-1-AP | Download protocol |
| IP protocol for XRCC4 antibody 15817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.
| ||
Cancer Res Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining. | ||
Cell Death Differ LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair. | ||
Toxicol Appl Pharmacol Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis. | ||
PLoS One 5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation. | ||
J Cancer Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma. |
