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验证数据展示

  • IHC staining of human prostate hyperplasia using 24410-1-AP

    IHC staining of human prostate hyperplasia using 24410-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24410-1-AP (ACPP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAP/ACPP antibody included
  • IHC staining of human prostate hyperplasia using 24410-1-AP

    IHC staining of human prostate hyperplasia using 24410-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24410-1-AP (ACPP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAP/ACPP antibody included

ACPP Polyclonal antibody

ACPP Polyclonal Antibody for IHC, ELISA
Cat No. 24410-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24410-1-AP targets ACPP in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 ACPP fusion protein Ag19701 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acid phosphatase, prostate
别名 acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase
计算分子量 418 aa, 48 kDa
GenBank蛋白编号BC007460
基因名称 ACPP
Gene ID (NCBI) 55
RRIDAB_2879529
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15309
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

实验方案

Product Specific Protocols
IHC protocol for ACPP antibody 24410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols