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ChromoTek anti-FGF-Basic recombinant VHH, biotinylated

bfgf-b is a recombinant mono-biotinylated anti FGF-basic (FGF2) Nanobody (VHH), suitable for ELISA, SPR, and BLI applications.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Cat No : bfgf-b

Synonyms

FGF-basic VHH, FGF2 VHH, FGF basic single domain antibody (sdAb), FGF2, FGF-2

Validation Data Gallery

  • BLI analysis of the capture of varying concentrations of HumanKine FGFbasic-TS (HZ-1285) by biotinylated anti-FGFbasic VHH (bfgf-b). Please note the higher apparent affinity owing to avidity effects (dimerization of the cytokine).

    BLI analysis of the capture of varying concentrations of HumanKine FGFbasic-TS (HZ-1285) by biotinylated anti-FGFbasic VHH (bfgf-b). Please note the higher apparent affinity owing to avidity effects (dimerization of the cytokine).

  • ELISA analysis of the capture of varying concentrations of HumanKine FGFbasic-TS (HZ-1285) by biotinylated anti-FGF-basic VHH (bfgf-b). Detection via mouse IgG1 PTG 69024-1-Ig.

    ELISA analysis of the capture of varying concentrations of HumanKine FGFbasic-TS (HZ-1285) by biotinylated anti-FGF-basic VHH (bfgf-b). Detection via mouse IgG1 PTG 69024-1-Ig.

Product Information

bfgf-b is a recombinant mono-biotinylated anti FGF-basic (FGF2) Nanobody (VHH), suitable for ELISA, SPR, and BLI applications.

ApplicationsBLI, SPR, ELISA
ReactivityHuman
ConjugateBiotin
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity4 nM
Molecular Weight14.5 kDa
FormLiquid
RRIDAB_3665409
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingambient
BackgroundBasic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGFbasic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGFbasic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).