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ChromoTek anti-FGF-Basic recombinant VHH, for 1x Cys conjugation

bfgfCys1 is an unconjugated recombinant anti FGF-basic (FGF2) Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Cat No : bfgfCys1

Synonyms

FGF-basic VHH, FGF2 VHH, FGF basic single domain antibody (sdAb), FGF2, FGF-2

Validation Data Gallery

  • BLI analysis of the interaction between varying concentrations of anti-FGFbasic-TS VHH and immobilised, biotinylated HumanKine FGFbasic-TS (HZ-1285). Fit indicates fitting of data to a 1:1 binding model.

    BLI analysis of the interaction between varying concentrations of anti-FGFbasic-TS VHH and immobilised, biotinylated HumanKine FGFbasic-TS (HZ-1285). Fit indicates fitting of data to a 1:1 binding model.

Product Information

bfgfCys1 is an unconjugated recombinant anti FGF-basic (FGF2) Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

ApplicationsConjugation
ReactivityHuman
ConjugateUnconjugated
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity4 nM
Molecular Weight14.5 kDa
FormLiquid
RRIDAB_3665410
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingdry ice
BackgroundBasic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGFbasic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGFbasic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).