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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

    WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

    IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with c-SRC antibody included
  • IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

    IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with c-SRC antibody included
  • WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

    WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 60315-1-Ig (same clone as 60315-1-PBS)

    IF Staining of MCF-7 using 60315-1-Ig (same clone as 60315-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using c-SRC antibody (60315-1-Ig, Clone: 5E10C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

c-SRC Monoclonal antibody, PBS Only

c-SRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60315-1-PBS

产品说明书

CloneNo. 5E10C4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, hamster

应用

WB, IHC, IF/ICC, ELISA

别名

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 
100 μg

¥5800/ 100 μg

浓度: 1mg/ml

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.

产品信息

60315-1-PBS targets c-SRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, hamster
免疫原 c-SRC fusion protein Ag22306 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
别名 SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC011566
基因名称 SRC
Gene ID (NCBI) 6714
ENSEMBL Gene IDENSG00000197122
RRIDAB_2881426
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12931
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.