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c-SRC Monoclonal antibody, PBS Only

c-SRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5E10C4

Cat no : 60315-1-PBS

Print datasheet

Synonyms

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

验证数据展示

  • WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

    WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

    IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with c-SRC antibody included
  • IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

    IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with c-SRC antibody included
  • WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

    WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 60315-1-Ig (same clone as 60315-1-PBS)

    IF Staining of MCF-7 using 60315-1-Ig (same clone as 60315-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using c-SRC antibody (60315-1-Ig, Clone: 5E10C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.

产品信息

60315-1-PBS targets c-SRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, hamster
Immunogen c-SRC fusion protein Ag22306 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Synonyms SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC011566
Gene Symbol SRC
Gene ID (NCBI) 6714
ENSEMBL Gene IDENSG00000197122
RRIDAB_2881426
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12931
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.