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human IgM Monoclonal antibody, PBS Only

human IgM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10B10

Cat no : 66484-1-PBS

Print datasheet

Synonyms

IgM, IGHM, VH, MU, 2D10B10

验证数据展示

  • WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

    WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

    0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.

产品信息

66484-1-PBS targets human IgM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen human IgM fusion protein Ag1459 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant mu
Synonyms IgM, IGHM, VH, MU, 2D10B10
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2881850
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).