• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

    WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

    0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:3200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:3200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgM antibody included
  • IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

human IgM Monoclonal antibody, PBS Only

human IgM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66484-1-PBS

产品说明书

CloneNo. 2D10B10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

IgM, IGHM, VH, MU, 2D10B10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.

产品信息

66484-1-PBS targets human IgM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 human IgM fusion protein Ag1459 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 immunoglobulin heavy constant mu
别名 IgM, IGHM, VH, MU, 2D10B10
计算分子量 69 kDa
观测分子量 75 kDa
GenBank蛋白编号BC009851
基因名称 IGHM
Gene ID (NCBI) 3507
RRIDAB_2881850
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).