ChromoTek iST Myc-Trap® IP-MS Sample Preparation Kit
Kit for IP/ Co-IP of Myc-tagged proteins & sample preparation for mass spectrometry (MS)
Cat No : ytak-iST
Synonyms
Validation Data Gallery
Product Information
Kit for IP/ Co-IP of Myc-tagged proteins & sample preparation for mass spectrometry (MS)
| Description | The iST Myc-Trap® Kit enables researchers to process Myc-tagged proteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek Myc-Trap for immunoprecipitation/Co-IP of Myc-tagged proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:
• Reliable Identification of Myc-tagged proteins & interacting partners by MS. Endogenous c-myc is NOT bound. • Identification of even low abundant/ low expressed Myc-tagged proteins • Superior processing of IP samples into clean peptide mixtures • Intuitive protocol and reproducible results |
| Applications | IP, MS |
| Specificity/Target | Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound. |
| Binding capacity | 17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | 1x Myc-tag: Dissociation constant KD of 500 nM 2x Myc-tag: Dissociation constant KD of 0.5 nM |
| Compatibility with mass spectrometry | The Myc-Trap® is optimized for on-bead digestion. |
| Storage Buffer | 20% ethanol |
| Storage Condition | Kit is shipped at ambient temperature. Upon receipt store Myc-Trap at 4°C and Digest at -20°C |
Kit components
| Component | Description |
|---|---|
| Myc-Trap Agarose | 8 reactions plus 2 controls |
| Digest | Enzyme Trypsin-mix to digest proteins. |
| Resuspend buffer | Protease reconstitution buffer for enzymes. |
| Lyse buffer | Denature, reduce and alkylate proteins. |
| Stop solution | Stop the enzymatic activity. |
| Wash 1 buffer | Clean up peptides from hydrophobic contaminants. |
| Wash 2 buffer | Clean up peptides from hydrophilic contaminants. |
| Elution buffer | Elute the peptides from the cartridge. |
| LC-Load load | Load peptides on reversed-phase LC-MS column. |
| Cartridges | Cartridge for 1 to 100 µg protein starting material. |
| Waste tubes | Tube for collecting waste after washing steps. |
| Collection tubes | Tube for collecting peptides after elution. |
| Adapters | Enables placing a cartridge into a tube. |
| Caps | Cap to optionally close the cartridge’s bottom. |
Documentation
| SDS |
|---|
| SDS iST Kits (grouped) EtOH (EN)-CTK |
| Protocol |
|---|
| Protocol ytak-iST_iST Myc-Trap Kit (PDF) |
Publications
| Application | Title |
|---|---|
bioRxiv ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate Cancer | |
Nucleic Acids Res ONECUT2 acts as a lineage plasticity driver in adenocarcinoma as well as neuroendocrine variants of prostate cancer |