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验证数据展示

  • WB analysis of HEK-293 using 66234-1-Ig (same clone as 66234-1-PBS)

    WB analysis of HEK-293 using 66234-1-Ig (same clone as 66234-1-PBS)

    WB result of p38 MAPK antibody (66234-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 MAPK transfected HEK-293 cells. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 66234-1-Ig (same clone as 66234-1-PBS)

    WB analysis of human heart using 66234-1-Ig (same clone as 66234-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with p38 MAPK antibody included
  • IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with p38 MAPK antibody included
  • WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66234-1-Ig (same clone as 66234-1-PBS)

    IF Staining of HepG2 using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p38 MAPK antibody (66234-1-Ig, Clone: 1A1C2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50419-1

    Cytometric bead array standard curve of MP50419-1

    Cytometric bead array standard curve of MP50419-1, p38 MAPK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66234-1-PBS. Detection antibody: 68965-1-PBS. Standard:Ag5797. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP50419-2

    Cytometric bead array standard curve of MP50419-2

    Cytometric bead array standard curve of MP50419-2, p38 MAPK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66234-1-PBS. Detection antibody: 68966-1-PBS. Standard:Ag5797. Range: 1.563-200 ng/mL.

  • Featured Product
  • KD/KO验证

p38 MAPK Monoclonal antibody, PBS Only (Capture)

p38 MAPK Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66234-1-PBS

产品说明书

CloneNo. 1A1C2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

产品信息

66234-1-PBS targets p38 MAPK as part of a matched antibody pair:

MP50419-1: 66234-1-PBS capture and 68965-1-PBS detection (validated in Cytometric bead array)

MP50419-2: 66234-1-PBS capture and 68966-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 p38 MAPK fusion protein Ag5797 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 14
别名 MAPK14, CSPB1, CSBP2, CSBP1, CSBP
计算分子量 360 aa, 41 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC031574
基因名称 p38 MAPK
Gene ID (NCBI) 1432
RRIDAB_2861335
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16539
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.