Phospho-TDP43 (Ser409/410) Polyclonal antibody

Phospho-TDP43 (Ser409/410) Polyclonal Antibody for WB, ELISA
Cat No. 22309-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, ELISA and More (3)

Phospho-TDP43, Phospho TDP43 (Ser409/410), Phospho TDP43, p TDP43 (Ser409/410), p TDP43

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22309-1-AP targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, monkey
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAR DNA binding protein
别名 Phospho-TDP43, Phospho TDP43 (Ser409/410), Phospho TDP43, p TDP43 (Ser409/410), p TDP43
计算分子量 43 kDa
观测分子量 40-50 kDa, 25-35 kDa
GenBank蛋白编号NM_007375
基因名称 TDP-43
Gene ID (NCBI) 23435
RRIDAB_11182943
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13148
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

实验方案

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 22309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Nat Med

The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.

Authors - Wenzhang Wang
humanWB

Nat Cell Biol

Caspase-2 is a condensate-mediated deubiquitinase in protein quality control

Authors - Yingwei Ge
mouseIHC,IF

Nat Neurosci

TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.

Authors - Manling Xie
human

Mol Neurodegener

TDP-43-regulated cryptic RNAs accumulate in Alzheimer's disease brains

Authors - Virginia Estades Ayuso
human

Alzheimers Dement

Entorhinal vessel density correlates with phosphorylated tau and TDP-43 pathology

Authors - Josué Llamas Rodríguez
humanIHC

JAMA Neurol

TDP-43 Accumulation Within Intramuscular Nerve Bundles of Patients With Amyotrophic Lateral Sclerosis.

Authors - Takashi Kurashige
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