验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
10018-3-AP targets Phospho-Marcksl1 (Ser93/104) in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | mouse |
| 文献引用反应性 | human, rat, bovine |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myristoylated alanine rich protein kinase C substrate |
| 别名 | Phospho-Marcksl1, MARCKS, MARCKS-like 1, Myristoylated alanine-rich C-kinase substrate, Marcksl1 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC046601 |
| 基因名称 | Marcks |
| Gene ID (NCBI) | 17118 |
| RRID | AB_2297345 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P26645 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated. Because of the sequence similarity, this antibody can recognize phospho-Marcksl1 and phospho-Marcks.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Marcksl1 (Ser93/104) antibody 10018-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability. | ||
J Neurosci NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia. | ||
J Physiol 5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase. | ||
Am J Physiol Cell Physiol Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability. | ||