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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human PDGFbb protein

Activity

0.3-3 ng/mL

Specific Activity

minimally 5.00 x 106 IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Cat No : HZ-1308

Print datasheet

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV

Validation Data Gallery

  • Purity of recombinant human PDGFbb was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human PDGFbb was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human PDGFbb (HZ-1308) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFbb for 48 hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.3-3 ng/mL.

    Recombinant human PDGFbb (HZ-1308) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFbb for 48 hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.3-3 ng/mL.

Technical Specifications

GeneID 5155
Species Human
Expression HEK293
Activity 0.3-3 ng/mL
Specific Activityminimally 5.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP31240
Molecular Mass 14 and 36 kDa reduced, 29 to 32 kDa non-reduced, homodimer, glycosylated
Formulation 50mM Acetate pH 3.5 + 500mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

PDGFbb is a member of the platelet-derived growth family factor. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFbb is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, and placenta among others. PDGFbb plays a role in blood vessel development. It also implements its role in wound healing (PMID: 29266203; 28851707; 28556368).

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV

Publications

SpeciesTitle
human

Br J Pharmacol

A novel HSF1 activator ameliorates nonalcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation

Authors - Yong Rao
View Article
mouse

J Neuroinflammation

SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway.

Authors - Ziyu Li
View Article
mouse

Front Pharmacol

Deletion of Smooth Muscle Lethal Giant Larvae 1 Promotes Neointimal Hyperplasia in Mice.

Authors - Ya Zhang
View Article
human

Arch Biochem Biophys

Quiescin/sulfhydryl oxidase 1b (QSOX1b) induces migration and proliferation of vascular smooth muscle cells by distinct redox pathways.

Authors - Karime C França
View Article
human

Commun Biol

KLF4-PFKFB3-driven glycolysis is essential for phenotypic switching of vascular smooth muscle cells

Authors - Xinhua Zhang
View Article

FASEB J

Deficiency of thrombospondin-2 alleviates intimal hyperplasia in mice by modulating vascular smooth muscle cell proliferation and migration

Authors - Xu Li
View Article