• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human PDGFbb protein

Activity

0.3-3 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1308

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV



Technical Specifications

GeneID 5155
Species Human
Expression HEK293
Activity 0.3-3 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP31240
Molecular Mass 29 to 32 kDa, homodimer, glycosylated
Formulation 50mM Acetate pH 3.5 + 500mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 10 mM HOAc containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

PDGFbb is a member of the platelet-derived growth family factor. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFbb is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, and placenta among others. PDGFbb plays a role in blood vessel development. It also implements its role in wound healing (PMID: 29266203; 28851707; 28556368).

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV

Publications

SpeciesTitle
human

Arch Biochem Biophys

Quiescin/sulfhydryl oxidase 1b (QSOX1b) induces migration and proliferation of vascular smooth muscle cells by distinct redox pathways.

Authors - Karime C França
mouse

Front Pharmacol

Deletion of Smooth Muscle Lethal Giant Larvae 1 Promotes Neointimal Hyperplasia in Mice.

Authors - Ya Zhang
mouse

J Neuroinflammation

SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway.

Authors - Ziyu Li
human

Br J Pharmacol

A novel HSF1 activator ameliorates nonalcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation

Authors - Yong Rao