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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human EPO protein

Activity

0.2-3.0 ng/mL

Specific Activity

minimally 3.0 x 105 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1168

Print datasheet

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2

Validation Data Gallery

  • Purity of recombinant human EPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human EPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human EPO (HZ-1168) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant EPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-3.0 ng/mL

    Recombinant human EPO (HZ-1168) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant EPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-3.0 ng/mL

Technical Specifications

GeneID 2056
Species Human
Expression HEK293
Activity 0.2-3.0 ng/mL
Specific Activityminimally 3.0 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP01588
Molecular Mass 36 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Erythropoietin (Epo) is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. Epo is found in the plasma and regulates erythrocyte production by promoting erythroid differentiation and initiating hemoglobin synthesis through activation of its cognate receptor (PMID 29458727). Epo also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types (PMID 11854521).

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2

Publications

SpeciesTitle
mouse

Nat Commun

Ulk1-mediated Atg5-independent macroautophagy mediates elimination of mitochondria from embryonic reticulocytes.

Authors - Shinya Honda
View Article
human

Stem Cells

Brief report: efficient generation of hematopoietic precursors and progenitors from human pluripotent stem cell lines.

Authors - Niels-BjarneWoods
View Article
human

Eur J Pharmacol

Ginsenoside Rg1 promotes fetal hemoglobin production in vitro: A potential therapeutic avenue for β-thalassemia

Authors - Dong-Ling Cai
View Article
mouse

Artif Cells Nanomed Biotechnol

EPO could be regulated by HIF-1 and promote osteogenesis and accelerate bone repair.

Authors - Yijun Yu
View Article
mouse

J Labelled Comp Radiopharm

99m Tc-labeled rHuEpo for imaging of the erythropoietin receptor in tumors.

Authors - XiaoqingZhuang
View Article