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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-7 (KGF) protein

Activity

4-20 ng/mL

Specific Activity

minimally 2.00 x 105 IU/mg

Species Reactivity

human,monkey

Purity

>95%

Cat No : HZ-1100

Print datasheet

Synonyms

FGF 7, FGF7, FGF-7, Fibroblast growth factor 7, HBGF 7, Keratinocyte growth factor, KGF

Validation Data Gallery

  • Purity of recombinant human FGF-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human FGF-7 (HZ-1100) Stimulates dose-dependent proliferation of the 4MBr-5 Monkey epithelial cell line. Viable cell number was quantitatively assessed by PrestoBlue Cell Viability Reagent. 4MBr-5 ells were treated with increasing concentrations of recombinant human FGF-7 for 120 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 4-20 ng/mL.

    Recombinant human FGF-7 (HZ-1100) Stimulates dose-dependent proliferation of the 4MBr-5 Monkey epithelial cell line. Viable cell number was quantitatively assessed by PrestoBlue Cell Viability Reagent. 4MBr-5 ells were treated with increasing concentrations of recombinant human FGF-7 for 120 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 4-20 ng/mL.

Technical Specifications

GeneID 2252
Species Human
Expression HEK293
Activity 4-20 ng/mL
Specific Activityminimally 2.00 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP21781
Molecular Mass 17 and 25 to 30 kDa reduced, 17 and 24 to 29 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,monkey

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Fibroblast growth factor 7 (FGF-7), also known as keratinocyte growth factor (KGF), belongs to the FGF family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion (PMID 11276432, 15863033). FGF-7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes (PMID 15327889).

Synonyms

FGF 7, FGF7, FGF-7, Fibroblast growth factor 7, HBGF 7, Keratinocyte growth factor, KGF

Publications

SpeciesTitle
human

J Invest Dermatol

Increased KGF expression promotes fibroblast activation in a double paracrine manner resulting in cutaneous fibrosis.

Authors - JohannaCanady
View Article
mouse

Stem Cell Res Ther

Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration.

Authors - Naoya Kawakita
View Article
human

Phytomedicine

Kaempferol inhibits SARS-CoV-2 invasion by impairing heptad repeats-mediated viral fusion

Authors - Junwei Gao
View Article

Exp Cell Res

Directed differentiation of human embryonic stem cells into conjunctival epithelial cells

Authors - Xiangyue Hu
View Article