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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN beta protein

Activity

0.015-0.08 ng/mL

Specific Activity

minimally 1.00 x 109 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1298

Print datasheet

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast

Validation Data Gallery

  • Purity of recombinant human IFN beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IFN beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IFN beta (HZ-1298) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.015-0.08 ng/mL​.

    Recombinant human IFN beta (HZ-1298) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.015-0.08 ng/mL​.

  • Proteintech IFN beta (HZ-1298) demonstrates equivalent induction of proliferation and EC50 to leading competitors. IFN beta dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.015-0.08 ng/mL​.

    Proteintech IFN beta (HZ-1298) demonstrates equivalent induction of proliferation and EC50 to leading competitors. IFN beta dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.015-0.08 ng/mL​.

Technical Specifications

GeneID 3456
Species Human
Expression HEK293
Activity 0.015-0.08 ng/mL
Specific Activityminimally 1.00 x 109 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01574
Molecular Mass 21 to 24 kDa reduced, 20 to 23 and 38 to 42 non-reduced, glycosylated
Formulation Sodium Acetate pH 4.8 + 150mM NaCl + CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile water. Gently swirl or tap vial to mix.

Background

IFN beta is a member of type I family of interferons. It binds to the IFNα/β receptor (IFNAR), activating the Jak/ STAT pathway. Activation of this signaling pathway results in upregulating genes that inhibit viral infection and regulate MHC class I antigens. It is primarily produced by fibroblasts and monocytes. In addition to inhibiting viral infection, IFN beta is also involved in regulating and activating immune response against bacteria, parasite and tumor cells. Multiple sclerosis is characterized by a deficiency of IFN beta 1. An injectable form of IFN beta 1 is used for MS treatment (PMID: 22222875; 29258190; 8602746; 8469318).

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast

Publications

SpeciesTitle
human

J Innate Immun

Positive Feedback Loop of Long Noncoding RNA OASL-IT1 and Innate Immune Response Restricts the Replication of Zika Virus in Epithelial A549 Cells.

Authors - Yi Wang
View Article
mouse

FEBS Lett

Linear ubiquitination-induced necrotic tumor remodeling elicits immune evasion

Authors - Katsuhiro Sasaki
View Article
human

Cell Rep

Critical role of Syk-dependent STAT1 activation in innate antiviral immunity.

Authors - Shasha Liu
View Article
human

Microbes Infect

MicroRNA-155 triggers a cellular antiviral immune response against Chandipura virus in human microglial cells

Authors - Neha Pandey
View Article
human

Arch Oral Biol

Ruxolitinib altered IFN-β induced necroptosis of human dental pulp stem cells during osteoblast differentiation

Authors - Atsuko Tanaka
View Article

Cell Rep

STAT3 regulates antiviral immunity by suppressing excessive interferon signaling

Authors - Shasha Liu
View Article