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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-17F protein

Activity

4.5-22.5 ng/mL

Species Reactivity

human

Purity

>95%

Cat No : HZ-1116

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Synonyms

Cytokine ML 1, IL 17F, IL17, IL-17, IL17F, IL-17F, interleukin 17F, ML 1, ML1

Validation Data Gallery

  • Purity of recombinant human IL-17F was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-17F was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-17F (HZ-1116) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 value range is 4.5-22.5 ng/mL.

    Recombinant human IL-17F (HZ-1116) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 value range is 4.5-22.5 ng/mL.

Technical Specifications

GeneID 112744
Species Human
Expression HEK293
Activity 4.5-22.5 ng/mL
Purity >95%
Endotoxin<1 EU/µg
Accession NumberQ96PD4
Molecular Mass 22 kDa reduced, 40 kDa non-reduced, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-17F is a homodimeric glycoprotein with an apparent molecular mass of 40 kDa. IL-17 is commonly associated with allergic responses. IL-17 induces the production of many other cytokines such as TNF alpha, chemokines such as IL-8 and prostaglandins. The IL17-induced release of cytokines has many effects, including airway remodeling in asthma (PMID 24292568)

Synonyms

Cytokine ML 1, IL 17F, IL17, IL-17, IL17F, IL-17F, interleukin 17F, ML 1, ML1