Purity of recombinant human SCF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human SCF (HZ-1024) stimulates dose-dependent proliferation of the MO7e human megakaryoblastic leukemia cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of GMP recombinant SCF for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 15-85 ng/mL​.