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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TGF beta 2 protein

Activity

0.018-0.18 ng/mL EC50

Specific Activity

3.52 x 107 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1092

Print datasheet

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2

Validation Data Gallery

  • Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human TGF beta 2 (HZ-1092) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.018-0.18 ng/mL.

    Recombinant human TGF beta 2 (HZ-1092) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.018-0.18 ng/mL.

Technical Specifications

GeneID 7042
Species Human
Expression HEK293
Activity 0.018-0.18 ng/mL EC50
Specific Activity3.52 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP27090
Molecular Mass 25 kDa, homodimer, non-glycosylated
Formulation 50 mM NaOAc pH 4.5, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 20% ethanol + 50 mM NaOAc + 75 mM HOAc.

Background

Transforming growth factor beta-2 (TGFB2) belongs to the TGF-beta family. TGFB2 is a secreted protein known as a cytokine that plays a vital role during embryonic development (PMID 19289080). TGFB2 regulates cell proliferation, differentiation, adhesion, migration by transducing the signal through transmembrane receptors type I and type II (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins (PMID 18313409). Disruption of the TGFB/SMAD pathway has been implicated in many cancers types. TGFB2 has suppressive effects on interleukin-2 dependent T-cell growth (PMID 12064833). The TGFβ2 protein plays a role in the blood vessels formation, the regulation of muscle tissue and body fat development. TGFβ-2 is especially abundant in tissues related to the skeleton, where it helps regulate bone growth and form the extracellular matrix in the spaces between cells.

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2

Publications

SpeciesTitle
mouse,human

J Cell Mol Med

FGF2 antagonizes aberrant TGFβ regulation of tropomyosin: role for posterior capsule opacity.

Authors - EriKubo
View Article
human

Biochem J

Soluble CD109 binds TGF-β and antagonizes TGF-β signalling and responses.

Authors - CarterLi
View Article
human

Biol Reprod

Constitutive activation of transforming growth factor Beta receptor 1 in the mouse uterus impairs uterine morphology and function.

Authors - YangGao
View Article
human

Invest Ophthalmol Vis Sci

Aldose Reductase Mediates Transforming Growth Factor β2 (TGF-β2)-Induced Migration and Epithelial-To-Mesenchymal Transition of Lens-Derived Epithelial Cells.

Authors - Kun-CheChang
View Article

Front Oncol

Association of the TGFβ gene family with microenvironmental features of gastric cancer and prediction of response to immunotherapy

Authors - Bangling Han
View Article