验证数据展示
经过测试的应用
| Positive ELISA detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 8 publications below |
产品信息
28867-1-AP targets SARS-CoV-2 S protein (944-1214 aa) in WB, IF, ELISA applications and shows reactivity with virus samples.
| 经测试应用 | ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | virus |
| 文献引用反应性 | human, monkey, hamster |
| 免疫原 | SARS-CoV-2 S protein (944-1214 aa) fusion protein Ag30685 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SARS-CoV-2 Spike Protein |
| 别名 | RBD, COVID-19 RBD, Spike, spike protein, SARS-CoV-2 Spike |
| 计算分子量 | 141 kDa |
| GenBank蛋白编号 | NC_045512 |
| 基因名称 | SARS-COV-2 |
| Gene ID (NCBI) | 43740568 |
| RRID | AB_2881223 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0DTC2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). This antibody detects the spike protein of SARS and SARS-COV-2.
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biomed Eng High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2 | ||
Nat Immunol Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. | ||
Cell Rep Diverse immunoglobulin gene usage and convergent epitope targeting in neutralizing antibody responses to SARS-CoV-2. | ||
J Virol The respective roles of TMPRSS2 and cathepsins for SARS-CoV-2 infection in human respiratory organoids |