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- KD/KO Validated
CDK2 Polyclonal antibody
CDK2 Polyclonal Antibody for WB, IP, IF/ICC,IF-P, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IP, IF/ICC,IF-P, IHC, ELISA and More (1)
Conjugate
Unconjugated
268
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, A431 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, rat kidney tissue, Hela cells, Jurkat cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, mouse testis tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10122-1-AP targets CDK2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IF/ICC,IF-P, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, sheep, sea cucumbers, fish, zebrafish |
| Immunogen | CDK2 fusion protein Ag0166 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin-dependent kinase 2 |
| Synonyms | CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2) |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC003065 |
| Gene Symbol | CDK2 |
| Gene ID (NCBI) | 1017 |
| RRID | AB_2078556 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24941 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK2 antibody 10122-1-AP | Download protocol |
| IHC protocol for CDK2 antibody 10122-1-AP | Download protocol |
| IF protocol for CDK2 antibody 10122-1-AP | Download protocol |
| IP protocol for CDK2 antibody 10122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
| ||
Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.
| ||
Sci Adv Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer | ||
Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. | ||
J Clin Invest TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. | ||
Nat Commun Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets. |
