Results

human BCL2 Polyclonal antibody

KO/KD Validated

2334 Publications

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A431 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, chicken, duck, fish, Goat, hamster, pig, rabbit, sheep, zebrafish

Applications

WB, IP, IF, IHC, CoIP, chIP, ELISA

Sizes
-_- / -_-

Cat No. 12789-1-AP

浓度：500 μg/ml
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A431 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-human BCL2 (IP:12789-1-AP, 4ug; Detection:12789-1-AP 1:1000) with HL-60 cells lysate 4000 ug.

WB result of BCL2 (12789-1-AP, 1:1000) with si-Control and si-BCL2 transfected Jurkat cells.
BCL2 Monoclonal antibodies (4H8C6) (5 total products)

279 Publications

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Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60178 (BCL2 antibody) at dilution of 1:100.

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, monkey, pig, rabbit

Applications

IF/ICC , IHC, IP/CoIP/ChIP/RIP

Conjugation

CoraLite® Plus 488, CoraLite®555, CoraLite®594, Unconjugated

CloneNo.

4H8C6
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Cat No. CL594-60178, CL488-60178, CL555-60178, 60178-1-Ig, 60178-1-PBS
Bcl2 Recombinant antibodies (240571H5) (2 total products)

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Various lysates were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

WB

Conjugation

Unconjugated

CloneNo.

240571H5
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Cat No. 82469-6-PBS, 82469-6-RR
Bcl2 Polyclonal antibodies (2 total products)

779 Publications

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Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Bcl2 antibody (CL488-26593) at dilution of 1:200.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, canine, chicken, duck, hamster, pig

Applications

IF/ICC , IP/CoIP/ChIP/RIP , WB

Conjugation

CoraLite® Plus 488, Unconjugated
-_- / -_-

Cat No. CL488-26593, 26593-1-AP
Bcl2 Monoclonal antibodies (1B3F7) (3 total products)

112 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Host/IsoType

Mouse/IgG1

Reactivity

mouse, rat, chicken

Applications

IF/ICC , IHC, WB

Conjugation

CoraLite® Plus 488, Unconjugated

CloneNo.

1B3F7
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Cat No. 68103-1-Ig, CL488-68103, 68103-1-PBS
Bcl2 Monoclonal antibodies (1B5B9) (3 total products)

19 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC , IP/CoIP/ChIP/RIP , WB

Conjugation

CoraLite®555, Unconjugated

CloneNo.

1B5B9
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Cat No. 66799-1-Ig, 66799-1-PBS, CL555-66799

Bcl2 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00608-1	82469-2-PBS	82469-4-PBS	Bcl2	Mouse	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00608-2	82469-3-PBS	82469-4-PBS	Bcl2	Mouse	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00608-3	82469-5-PBS	82469-4-PBS	Bcl2	Mouse	Cytometric bead array	CBA: 0.313-40 ng/mL

BCL2 Recombinant antibodies (3O5) (6 total products)

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Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 647 BCL2 antibody (CL647-80313, Clone: 3O5 ) at dilution of 1:200.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

IF/ICC , IHC, WB

Conjugation

CoraLite® Plus 488, CoraLite® Plus 647, CoraLite®555, CoraLite®594, Unconjugated

CloneNo.

3O5
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Cat No. CL647-80313, 80313-1-RR, 80313-1-PBS, CL594-80313, CL488-80313, CL555-80313
IHCeasy BCL2 Ready-To-Use IHC Kit

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Primary Ab Type

Mouse Monoclonal

Reactivity

human

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Mouse

Sizes
-_- / -_-

Cat No. KHC0012
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More validation images available on the product page

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using KHC0012 (BCL2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using KHC0012 (BCL2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using KHC0012 (BCL2 IHC Kit).
Phospho-BCL2 (Ser70) Recombinant antibodies (230061A8) (2 total products)

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Non-treated Jurkat cells, paclitaxel treated Jurkat cells, non-treated Jurkat cells and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB

Conjugation

Unconjugated

CloneNo.

230061A8
-_- / -_-

Cat No. 80771-2-RR, 80771-2-PBS
BCL2 E.Coli expressed Tagged-proteins (5 total products)

3 Publications

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Species

human, mouse

Source

PET28a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag24260, Ag26118, Ag27874, Ag29064, Ag3508
Recombinant PLK3 (57-340) protein

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Expressed In

Baculovirus

Protein species

Human

Sizes
-_- / -_-

Cat No. 81372
×

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More validation images available on the product page

Recombinant PLK3 (57-340) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 33.7 kDa Purity: >85%

HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

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研究领域

human BCL2 Polyclonal antibody

-_- / -_-

BCL2 Monoclonal antibodies (4H8C6) (5 total products)

-_- / -_-

Bcl2 Recombinant antibodies (240571H5) (2 total products)

-_- / -_-

Bcl2 Polyclonal antibodies (2 total products)

-_- / -_-

Bcl2 Monoclonal antibodies (1B3F7) (3 total products)

-_- / -_-

Bcl2 Monoclonal antibodies (1B5B9) (3 total products)

-_- / -_-

Bcl2 Matched Antibody Pairs^New

BCL2 Recombinant antibodies (3O5) (6 total products)

-_- / -_-

IHCeasy BCL2 Ready-To-Use IHC Kit

-_- / -_-

Phospho-BCL2 (Ser70) Recombinant antibodies (230061A8) (2 total products)

-_- / -_-

BCL2 E.Coli expressed Tagged-proteins (5 total products)

-_- / -_-

Recombinant PLK3 (57-340) protein

-_- / -_-

human BCL2 Polyclonal antibody

-_- / -_-

BCL2 Monoclonal antibodies (4H8C6) (5 total products)

-_- / -_-

Bcl2 Recombinant antibodies (240571H5) (2 total products)

-_- / -_-

Bcl2 Polyclonal antibodies (2 total products)

-_- / -_-

Bcl2 Monoclonal antibodies (1B3F7) (3 total products)

-_- / -_-

Bcl2 Monoclonal antibodies (1B5B9) (3 total products)

-_- / -_-

Bcl2 Matched Antibody PairsNew

BCL2 Recombinant antibodies (3O5) (6 total products)

-_- / -_-

IHCeasy BCL2 Ready-To-Use IHC Kit

-_- / -_-

Phospho-BCL2 (Ser70) Recombinant antibodies (230061A8) (2 total products)

-_- / -_-

BCL2 E.Coli expressed Tagged-proteins (5 total products)

-_- / -_-

Recombinant PLK3 (57-340) protein

-_- / -_-

Products derived from

Bcl2 Matched Antibody Pairs^New