1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human ALCAM (CL405-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5ul CoraLite® Plus 405 Anti-Human ALCAM. Cells were not fixed. Monocytes were gated.
1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite®405 Anti-Human CD107a / LAMP1 (CL405-65051, Clone:H4A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite®405 Anti-Human CD107a / LAMP1 (CL405-65051, Clone:H4A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD11a (CL405-65194, Clone:TS2/4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD11a (CL405-65194, Clone:TS2/4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD11b (CL405-65116, Clone: ICRF44) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD11b (CL405-65116, Clone: ICRF44) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite®405 Anti-Human CD11c (CL405-65086, Clone:3.9) or Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite®405 Anti-Human CD11c (CL405-65086, Clone:3.9) or Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD11c (CL405-65196, Clone:BU15) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD146 (CL405-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD146 (CL405-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 human PBMCs were surface co-stained with PE Anti-Human CD3 and 5 ul CoraLite®405 Anti-Human CD16 (CL405-65090, Clone:3G8) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3- lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with PE Anti-Human CD3 and 5 ul CoraLite®405 Anti-Human CD16 (CL405-65090, Clone:3G8) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.