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Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.
