Human PIGF ELISA Kit
Validation Data Gallery
Product Information
KE00173 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PIGF ELISA kit is to be used to detect and quantify protein levels of endogenous PIGF. The assay recognizes human PIGF. An antibody specific for PIGF has been pre-coated onto the microwells. The PIGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for PIGF is added to detect the captured PIGF protein.For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human PIGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 16.8 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5228 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 88% | 70%-118% |
| Human serum | 83% | 72%-100% |
| cell culture supernatants | 92% | 74%-110% |
| Urine | 90% | 80%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4,631 | 272.8 | 5.9 |
| 2 | 20 | 1,009 | 62.7 | 6.2 |
| 3 | 20 | 238 | 14.2 | 5.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4,095 | 307.0 | 7.5 |
| 2 | 24 | 959 | 54.1 | 5.6 |
| 3 | 24 | 230 | 12.9 | 5.6 |
Background Information
PlGF (placenta growth factor), also named as PGF, is an angiogenic factor which belongs to vascular endothelial growth factor (VEGF) family. The human PlGF gene is located on chromosome 14q14 and encodes 4 isoforms of PlGF. The protein is secreted as a glycosylated homodimer and PlGF-1 and -3 are diffusible isoforms whereas PlGF-2 and PlGF-4 have heparin binding domains. PlGF was originally identified in the placenta, where it regulates growth and differentiation of trophoblasts. It is also expressed in umbilical vein endothelial cells and other non-placental tissues, like the thyroid gland and developing lung tissue. PlGF has angiogenic properties, enhancing survival, growth and migration of endothelial cells in vitro, and promotes vessel formation in certain in-vivo models. Serum levels of PIGF and sFlt-1 (also known as soluble VEGF receptor-1) are altered in women with preeclampsia. PIGF is a potential biomarker for preeclampsia, a condition in which blood vessels in the placenta are too narrow, resulting in high blood pressure.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PGF, PGFL, PIG F, PIGF, Placenta growth factor |
Publications
| Species | Sample Type | Title |
|---|---|---|
Toxicol Appl Pharmacol VEGFR1 TK signaling protects the lungs against LPS-induced injury by suppressing the activity of alveolar macrophages and enhancing the anti-inflammatory function of monocyte-derived macrophages |