Mouse EPO ELISA Kit

Sensitivity

12 pg/mL

Range

78.1-5000 pg/mL

Reactivity

Mouse

Cat No : KE10031

说明书下载

Synonyms

Epo, erythropoietin



Product Information

KE10031 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Erythropoietin ELISA kit is to be used to detect and quantify protein levels of endogenous Erythropoietin. The assay recognizes mouse Erythropoietin. An antibody specific for Erythropoietin has been pre-coated onto the microwells. The Erythropoietin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Erythropoietin is added to detect the captured mouse Erythropoietin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse EPO ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 12 pg/mL
Range 78.1-5000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 13856

Recovery

Sample TypeAverageRange
Mouse serum90%71%-117%
cell culture supernatants98%70%-119%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120252.220.78.2
2201,053.847.44.5
3204,276.6197.04.6

InterAssay

Samplenmean ( pg/mL)SDCV%
124267.722.88.5
2241,074.648.04.5
3244,481.0204.84.6

Background Information

Erythropoietin (EPO) is a glycoprotein hormone that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults, and and the secreted protein will travel through the blood stream to reach to the bone marrow to stimulate hematopoietic stem cell differentiation to RBC. EPO binds to the cognate EPO receptor (EPOR) on erythroid progenitor cells, thus preventing apoptosis and stimulating their differentiation and maturation into erythrocyte. However, EPO protein and its receptors have also been shown to be cytoprotective in extra-hematopoietic tissues including the retina tissue. A complete lack of erythropoietin causes embryonic lethal anemia in mice. The conditional inactivation of erythropoietin in adult mice results in a chronic, normocytic and normochromic anemia. 

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Epo, erythropoietin

Publications

SpeciesSample TypeTitle
mouseserum

Nutrition

Changes in iron metabolism centered on hepcidin due to high-intensity exercise under restricted food intake.

Authors - Yukiko Kobayashi
mouseplasma

ACS Pharmacol Transl Sci

Prolyl Hydroxylase Domain Protein Inhibitor Not Harboring a 2-Oxoglutarate Scaffold Protects against Hypoxic Stress.

Authors - Kento Sonoda