Various lysates were subjected to SDS PAGE followed by western blot with 83288-2-RR (NUP85 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NUP85 Recombinant antibody (83288-2-RR, Clone:240116E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP00330-1, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-1-PBS. Standard: Ag7086. Range: 0.313-40 ng/mL.
Cytometric bead array standard curve of MP00330-2, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-3-PBS. Standard: Ag7086. Range: 1.25-40 ng/mL.