Western blot analysis of cell extract from HEK293T cells transiently expressing SNAP-Tubulin and from untransfected cells.

Sensitivity tested with purified recombinant SNAP-tag protein

Pull-down of SNAP-tag protein with using SNAP/CLIP-tag-Trap Agarose.I: Input, FT: Flow-through, B: Bound.

Structure of SNAP/CLIP-tag.

Pull-down of SNAP-tag protein with using SNAP/CLIP-tag-Trap Agarose. I: Input, FT: Flow-through, B: Bound.

mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WB result of CLIP-115/CLIP2 antibody (67776-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CLIP-115/CLIP2 antibody (67776-1-Ig, Clone: 2E6B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 25088-1-AP (CLIP-115/CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WB result of CLIP-115/CLIP2 antibody (25088-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 25088-1-AP (CLIP-115/CYLN2 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-CLIP-115/CLIP2(IP:25088-1-AP, 4ug; Detection:25088-1-AP 1:15000) with mouse brain tissue lysate 1080 ug.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25088-1-AP (CLIP-115/CLIP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1x10^6 PC-12 cells were intracellularly stained with 0.25 ug SNAP25 Recombinant antibody (83259-1-RR, Clone:240069A1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using SNAP antibody (83259-1-RR, Clone: 240069A1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67776-1-PBS in a different storage buffer formulation.

WB result of CLIP-115/CLIP2 antibody (67776-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells. This data was developed using the same antibody clone with 67776-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CLIP-115/CLIP2 antibody (67776-1-Ig, Clone: 2E6B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67776-1-PBS in a different storage buffer formulation.