Non-transfected and transfected HEK-293T lysates were subjected to SDS PAGE followed by western blot with HRP-81290 (HA tagged antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Human plasma were subjected to SDS PAGE followed by western blot with HRP-11449 (Human IgA Heavy Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP Result of anti-alpha Tubulin (IP: 66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug. SA00001-2 (HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) ) as the secondary antibody.
Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. SA00001-1 (HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) as secondary antibody.
Western blot analysis of beta actin in various tissues and cell lines using Proteintech antibody HRP-60008 at a dilution of 1:5000.
HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60008 (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66005 (6*His, His-Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IgG proteins of various species were subjected to SDS PAGE followed by western blot with SA00001-4 (HRP-conjugated Affinipure Rabbit Anti-Goat IgG(H+L) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with Biotin-60293 (OPTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SA00001-0 (HRP-conjugated Streptavidin) as secondary antibody, at dilution of 1:3000 (lane 1 and 3) or 1:6000 (lane 2 and 4).
IgG proteins of various species were subjected to SDS PAGE followed by western blot with SA00001-3 (HRP-conjugated Affinipure Donkey Anti-Goat IgG(H+L) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with HRP-66009 (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IgG proteins of various species were subjected to SDS PAGE followed by western blot with SA00001-17 (HRP-conjugated Affinipure Goat Anti-Human IgG(H+L) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66001 (GST Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP result of anti-RCC1 (IP:10697-1-AP, 4ug; Detection:10697-1-AP 1:500) with HeLa cells lysate 1600 ug. SA00001-7L (HRP-Mouse Anti-Rabbit IgG Light Chain Specific) as secondary antibody.
Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66008 (DYKDDDDK Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.