Immunoprecipitation with mNeonGreen-Trap Magnetic Agarose: Coomassie and Western blot I: Input, FT: Flow-through, B: Bound.

Immunoprecipitation of mNeonGreen:Coomassie and Western Blot; Western blot indicates high effectiveness of pull-down.I: Input, FT: Flow-through, B: Bound.

Immunoprecipitation of mNeonGreen:Coomassie and Western Blot; Western blot indicates high effectiveness of pull-down.I: Input, FT: Flow-through, B: Bound.

Make the most of your time & gain about 40 hours with the iST mNeonGreen-Trap Kit.

Schematic workflow of iST_mNeonGreen -Tap_kit.

Preomics Technology Priciple.

Immunoprecipitation with mNeonGreen-Trap Magnetic Agarose: Coomassie and Western blot I: Input, FT: Flow-through, B: Bound.

mNeonGreen tagged proteins and Native HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 29523-1-AP (mNeonGreen antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Jurkat cells were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunostaining of Drosphila embryos expressing an mNeonGreen tagged protein. Left mNeonGreen, right anti-mNeonGreen antibody 32F6. Corutesy Clara Sidor, MRC-LMB

Immunostaining of HeLa cells transiently expressing mNeonGreen fused to Actin Chromobody (green) with 32F6 anti-mNeonGreen antibody (red). Merge image shows overlay of green and red channels and DAPI (blus). Scale bar, 10 μm