Various lysates were subjected to SDS PAGE followed by western blot with 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of HepG2 cells using Ribosomal protein L4 antibody (11302-1-AP) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Ribosomal protein L4 (11302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB result of Ribosomal protein L4 antibody (11302-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HeLa cells.
IP result of anti-Ribosomal protein L4 (IP:11302-1-AP, 4ug; Detection:11302-1-AP 1:6000) with HeLa cells lysate 1050 ug.