ChromoTek SARS-CoV-2 Spike Recombinant VHH [NM1221]

Specificity

Virus

Applications

BLI, Multiplex ACE2 competition assay

Conjugate

Unconjugated

Cat No : sc-NM1221

Synonyms

SARS-CoV-2, SARS-CoV-2 Spike, Spike

BLI binding kinetics of SARS-CoV-2 Spike Recombinant VHH [NM1221] to RBD. Biotinylated RBD was immobilized on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of SARS-CoV-2 Spike Recombinant VHH [NM1221] (ChromoTek sc-NM1221).

SKU/Size

Recombinant monovalent Nanobody against the Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein

Specificity	Virus
Host	Alpaca, recombinantly produced
Clonality	Monoclonal
Clone No.	NM1221 [Wagner et al. EMBO Rep. 2021 May 5;22(5):e52325. PMID: 33904225]
Class	Recombinant
Type	Nanobody / VHH, monovalent
Epitope	Amino acids 432-452 and 487-496 (determined by HDX-MS)
RRID	AB_2892247
Immunogen	RBD of SARS-CoV-2 Spike protein
Full Name	SARS-CoV-2 Spike protein
Calculated MW	25 kDa
GenBank Accession Number	NC_045512
Gene Symbol	COVID-19 S Protein
GeneID (NCBI)	43740568
Affinity (K_D)	27 nM
Conjugate	Unconjugated
Form	Liquid
Purification Method	Recombinant expression, affinity purification by IMAC. Note: SARS-CoV-2 Spike Recombinant VHH carries a C-terminal Myc-tag and His-tag.
Concentration	1000 µg/mL (65.1 µM)
Positive Controls	BLI: biotinylated RBD and SARS-CoV-2 Spike Recombinant VHH
Cited Applications	BLI, Multiplex ACE2 competition assay Note: Not recommended for Western blotting
Storage Buffer	PBS, 0.09% sodium azide Safety datasheet (SDS): sodium azide
Storage Condition	Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.
Stability	Stable for 1 year at -20°C/-4°F after shipment.
Shipment	Shipped at ambient temperature.

SDS
SDS SARS-CoV-2 Spike Recombinant VHH [NM1221] (PDF)

Protocol
Protocol SARS-CoV-2 Spike Recombinant VHH [NM1221] PDF