ChromoTek SARS-CoV-2 Spike Recombinant VHH [NM1267]

Recombinant bivalent Nanobody against the Receptor-binding domain (RBD) of SARS- CoV-2 Spike protein

Specificity

Virus

Applications

BLI, Multiplex ACE2 competition assay

Conjugate

Unconjugated

Cat No : sc-NM1267

Synonyms

SARS-CoV-2, SARS-CoV-2 Spike, Spike



Product Information

Recombinant bivalent Nanobody against the Receptor-binding domain (RBD) of SARS- CoV-2 Spike protein

SpecificityVirus
ClonalityMonoclonal
HostAlpaca, recombinantly produced
ClassRecombinant
Clone No.NM1267 (NM1230-NM1226 fusion)
[Wagner et al. EMBO Rep. 2021 May 5;22(5):e52325. PMID: 33904225]
TypeNanobody / VHH, biparatopic
RRIDAB_2892257
EpitopeNM1230: Amino acids 351 and 437-503 (determined by X-Ray crystallography)
NM1226: Amino acids 369-384 and 405, 408 and 504-508 (determined by HDX-MS)
ImmunogenRBD of SARS-CoV-2 Spike protein
Full NameSARS-CoV-2 Spike Protein
Calculated MW25 kDa
GenBank Accession NumberNC_045512
Gene SymbolCOVID-19 S Protein
GeneID (NCBI)43740568
ConjugateUnconjugated
Affinity (KD) NM1230: 9.5 nM
NM1226: 7.0 nM
FormLiquid
Purification MethodRecombinant expression, affinity purification by IMAC.
Note: SARS-CoV-2 Spike Recombinant VHH carries a C-terminal His-tag.
Concentration1000 µg/mL (34.9 µM)
Positive ControlsBLI: biotinylated RBD and SARS-CoV-2 Spike Recombinant VHH
Cited ApplicationsBLI, Multiplex ACE2 competition assay
Note: Not recommended for Western blotting
Storage BufferPBS, 0.09% sodium azide
Safety datasheet (SDS): sodium azide
Storage ConditionAliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.
StabilityStable for 1 year at -20°C/-4°F after shipment.
ShipmentShipped at ambient temperature.