Recombinant spike protein (RBD domain) were subjected to SDS PAGE followed by western blot with 67758-1-lg (SARS-CoV-2 Spike Glycoprotein (319-541 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Surrogate virus neutralization test of SARS-CoV-2 Spike Glycoprotein (319-541 aa) monoclonal antibody 67758-1-Ig using commercial kit. Briefly, RBD protein has been pre-coated on microplate, HRP labeled ACE2 protein and serial dose of 67758-1-Ig are added to the plate simultaneously and incubated for 1 hour at 37°C. The plate was then washed and signal was developed by adding chromogenic substrate followed by stop buffer. Signal strength was monitored at absorbance of 450nm. The IC50 is around 20 μg/mL. Please note that different IC50 will be obtained if different assays are used. Lyophilized format of this product is available.

SARS-CoV-2 Spike Antibody (28867-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (28867-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Recombinant protein were subjected to SDS PAGE followed by western blot with 82734-4-RR (spike protein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SARS-CoV-2 Spike Antibody (28869-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (28869-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

E.coli expressed SARS-CoV-2 spike protein (Cat.NO. Ag30688) was subjected to SDS PAGE followed by western blot with 28991-1-AP (SARS-CoV-2 S protein (319-541 aa) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP) tested by ELISA. SARS-CoV-2 Spike Glycoprotein (319-541 aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

Spike Glycoprotein with the domain 1-658aa expressed in HEK-293 cells were subjected to SDS PAGE followed by western blot with 28901-1-AP (SARS-CoV-2 Spike Glycoprotein (428-506 aa) Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SARS-CoV-2 Spike Glycoprotein (428-506aa) Antibody (28901-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein (428-506aa) Antibody (28901-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. .

Spike Glycoprotein (944-1214 aa) were subjected to SDS PAGE followed by western blot with 67794-1-lg (SARS-CoV-2 Spike Glycoprotein (944-1214 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Spike Glycoprotein (944-1214 aa) were subjected to SDS PAGE followed by western blot with 67794-1-lg (SARS-CoV-2 Spike Glycoprotein (944-1214 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67794-1-PBS in a different storage buffer formulation.