40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong).

Various lysates were subjected to SDS PAGE followed by western blot with 10782-2-AP (TDP-43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (10782-2-AP) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of TDP-43 antibody (10782-2-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-TDP-43 (IP:10782-2-AP, 4ug; Detection:10782-2-AP 1:50000) with HeLa cells lysate 1520 ug.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (10782-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

IHC results of Phospho-TDP43 (Ser409/410) rabbit recombinant antibody (80007-1-RR, 1000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann.

It's human subiculum (high-mag) and temporal cortex (medium-mag) from subject with limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Staining provided by Pete Nelson and Ela Patel, U. Kentucky AD Research Center Neuropathology Core.

HeLa cells were subjected to SDS PAGE followed by western blot with 80007-1-RR (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

1X10^6 Jurkat cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-TDP43 (Ser409/410) (80007-1-RR, Clone:6M10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 , or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong).

IP Result of anti-TDP-43 (human specific) (IP:60019-2-Ig, 5ug; Detection:60019-2-Ig 1:1000) with K-562 cells lysate 1720ug.

1X10^6 MCF-7 cells were stained with 0.2ug TDP-43 (human specific) antibody (60019-2-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Non-treated and Calyculin A treated HeLa cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

Various cell lysates were subjected to SDS PAGE followed by western blot with 80002-1-RR (TDP-43 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80002-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

WB result of TDP-43 antibody (80002-1-RR; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

HAP1 lysates prepared and IP of TARDBP performed using 2.0 μg of 80002-1-RR coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80002-1-RR at 1:200 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (for IF/FC) (80002-1-RR, Clone:16A22) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Various cell lysates were subjected to SDS PAGE followed by western blot with 80001-1-RR (TDP43 antibody) at dilution of 1:35000 incubated at room temperature for 1.5 hours.

WB result of TDP-43 antibody (80001-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80001-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80001-1-RR (TDP-43 antibody) at dilution of 1:150 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80001-1-RR at 1:400 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC results of TDP43 rabbit recombinant antibody (80001-1-RR, 1:20000) with the spinal cord slides from ALS patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann.

IHC results of TDP43 rabbit recombinant antibody (80001-1-RR, 1:20000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:2000 (under 4x lens).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (80001-1-RR, Clone:11N20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded Jurkat (left) and calyculin A treated Jurkat (right) cells slide using KHC0291 (Phospho-TDP43 (Ser409/410) IHC Kit).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using KHC0001 (TDP-43 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0001 (TDP-43 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0001 (TDP-43 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using KHC0001 (TDP-43 IHC Kit).