Make the most of your time & gain about 40 hours with the iST-V5-Trap Kit.

Schematic workflow of iST-V5-Trap Kit.

Preomics Technology Priciple.

V5-Trap® Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-Through, B: Bound.

V5-Trap® Agarose for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 488 I: Input, FT: Flow-Through, B: Bound.

V5-Trap® Agarose for pull-down. The V5-tag is N-terminal. I: Input, FT: Flow-Through, B: Bound.

IP from different cell lysates without V5-tagged proteins => The V5-Trap® has very low background.

V5-Trap® Magnetic Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-through, B: Bound.

Pulldown of V5-tagged protein (V5-tag at C-terminus) with V5-Trap® Magnetic Agarose beads, Coomassie and Western blot. Western blot: V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse IgG1 Nano-Secondary Alexa Fluor® 488 I: input, FT: Flow-through, B: Bound.

V5-Trap® Magnetic Agarose for pull-down of V5-tagged proteins. V5 at the N-terminus. I: Input, FT: Flow-through, B: Bound.

Very low background of V5-Trap® Magnetic Agarose: IP from different cell lysates without V5-tagged protein.

V5-Trap® Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-Through, B: Bound.

V5-Trap® Agarose for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 488 I: Input, FT: Flow-Through, B: Bound.

V5-Trap® Agarose for pull-down. The V5-tag is N-terminal. I: Input, FT: Flow-Through, B: Bound.

IP from different cell lysates without V5-tagged proteins => The V5-Trap® has very low background.

V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. I: Input, FT: Flow-Through, B: Bound.

V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

V5-Trap® Magnetic Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-through, B: Bound.

Pulldown of V5-tagged protein (V5-tag at C-terminus) with V5-Trap® Magnetic Agarose beads, Coomassie and Western blot. Western blot: V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse IgG1 Nano-Secondary Alexa Fluor® 488 I: input, FT: Flow-through, B: Bound.

V5-Trap® Magnetic Agarose for pull-down of V5-tagged proteins. V5 at the N-terminus. I: Input, FT: Flow-through, B: Bound.

Very low background of V5-Trap® Magnetic Agarose: IP from different cell lysates without V5-tagged protein.

V5-Trap® Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound.

V5-Trap Magnetic Particles M-270 for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. I: Input, FT: Flow-Through, B: Bound

Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.

IP result of anti-V5-tag (IP:14440-1-AP, 3ug; Detection:14440-1-AP 1:20000) with Recombinant protein protein lysate 3000ug.