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ACAT1 Polyclonal antibody
ACAT1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16215-1-AP targets ACAT1 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | ACAT1 fusion protein Ag9215 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | acetyl-Coenzyme A acetyltransferase 1 |
Synonyms | ACAT, ACAT1, Acetoacetyl CoA thiolase, MAT, T2, THIL |
Calculated Molecular Weight | 45 kDa |
Observed Molecular Weight | 38-45 kDa |
GenBank Accession Number | BC010942 |
Gene Symbol | ACAT1 |
Gene ID (NCBI) | 38 |
RRID | AB_2220210 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P24752 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ACAT1 antibody 16215-1-AP | Download protocol |
IHC protocol for ACAT1 antibody 16215-1-AP | Download protocol |
IF protocol for ACAT1 antibody 16215-1-AP | Download protocol |
IP protocol for ACAT1 antibody 16215-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
Hum Mol Genet Partial Complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage. | ||
Cell Mol Gastroenterol Hepatol S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis. | ||
BMC Med Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway. |