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CoraLite® Plus 488-conjugated ACAT1 Polyclonal antibody
ACAT1 Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ACAT1 Ready-To-Use IHC Kit. ACAT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16215-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-16215 targets ACAT1 in IF applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | ACAT1 fusion protein Ag9215 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | acetyl-Coenzyme A acetyltransferase 1 |
Synonyms | ACAT, ACAT1, Acetoacetyl CoA thiolase, MAT, T2, THIL |
Calculated Molecular Weight | 45 kDa |
Observed Molecular Weight | 38-45 kDa |
GenBank Accession Number | BC010942 |
Gene Symbol | ACAT1 |
Gene ID (NCBI) | 38 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P24752 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 ACAT1 antibody CL488-16215 | Download protocol |
FC protocol for CL Plus 488 ACAT1 antibody CL488-16215 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |