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验证数据展示

  • IF Staining of HeLa using CL488-16215

    IF Staining of HeLa using CL488-16215

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ACAT1 antibody (CL488-16215) at dilution of 1:200.

  • FC experiment of HeLa using CL488-16215

    FC experiment of HeLa using CL488-16215

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ACAT1 (CL488-16215) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ACAT1 Polyclonal antibody

ACAT1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-16215

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

MAT, EC:2.3.1.9, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, Acetoacetyl CoA thiolase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥3200/ 100 μL

浓度: 1000 μg/ml

库存：现货

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ACAT1 Ready-To-Use IHC Kit. ACAT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16215-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16215 targets ACAT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ACAT1 fusion protein Ag9215 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acetyl-Coenzyme A acetyltransferase 1
Synonyms MAT, EC:2.3.1.9, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, Acetoacetyl CoA thiolase
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 38-45 kDa
GenBank Accession NumberBC010942
Gene Symbol ACAT1
Gene ID (NCBI) 38
RRIDAB_3083985
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24752
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACAT1 antibody CL488-16215Download protocol
FC protocol for CL Plus 488 ACAT1 antibody CL488-16215Download protocol
Standard Protocols
Click here to view our Standard Protocols