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BMI1 Monoclonal antibody

BMI1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66161-1-Ig

产品说明书

CloneNo. 2E10A11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, monkey

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

RNF51, Polycomb group RING finger protein 4, Polycomb complex protein BMI-1, PCGF4, 2E10A11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-12 cells, HT-1080 cells, U2OS cells, U-937 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, COS-7 cells
Planning an IHC experiment? We recommend our IHCeasy BMI1 Ready-To-Use IHC Kit. BMI1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66161-1-Ig targets BMI1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, monkey samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, monkey
文献引用反应性human, mouse
免疫原 BMI1 fusion protein Ag21284 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 BMI1 polycomb ring finger oncogene
别名 RNF51, Polycomb group RING finger protein 4, Polycomb complex protein BMI-1, PCGF4, 2E10A11
计算分子量 37 kDa
观测分子量37-43 kDa
GenBank蛋白编号BC011652
基因名称 BMI1
Gene ID (NCBI) 648
RRIDAB_2881557
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35226
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.

实验方案

Product Specific Protocols
WB protocol for BMI1 antibody 66161-1-IgDownload protocol
IHC protocol for BMI1 antibody 66161-1-IgDownload protocol
IF protocol for BMI1 antibody 66161-1-IgDownload protocol
IP protocol for BMI1 antibody 66161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai
humanWB

Autophagy

Inhibition of BMI1 induces autophagy-mediated necroptosis.

Authors - Anindya Dey
  • KD Validated
mouseWB

Clin Transl Med

Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.

Authors - Haiyun Chen
mouseWB

J Bone Miner Res

Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription

Authors - Qiuyi Wang
humanWB

Aging (Albany NY)

Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.

Authors - Zhengwei Zhang
human,mouseWB,IHC

Mol Cancer Ther

Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.

Authors - Anindya Dey
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