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  • Featured Product
  • KD/KO Validated

FIS1 Polyclonal antibody

FIS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10956-1-AP

Print datasheet

Synonyms

TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135

验证数据展示

  • WB analysis using 10956-1-AP

    WB analysis using 10956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa cells using 10956-1-AP

    WB analysis of HeLa cells using 10956-1-AP

    WB result of FIS1 antibody (10956-1-AP, 1:8,000) with si-Control and si-FIS1 transfected HeLa cells.

  • IF Staining of Hepa1-6 cells using 10956-1-AP

    IF Staining of Hepa1-6 cells using 10956-1-AP

    IF result of anti-FIS1(10956-1-AP,1:100) with Hepa1-6 cell by Dr. Steven Eugene Smith. Mitochondrion outer membrane (Green) Stain.

  • IF Staining of HepG2 using 10956-1-AP

    IF Staining of HepG2 using 10956-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10956-1-AP (FIS1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of rat brain using 10956-1-AP

    IHC staining of rat brain using 10956-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10956-1-AP (FIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 10956-1-AP

    WB analysis using 10956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10956-1-AP

    IP experiment of HeLa using 10956-1-AP

    IP result of anti-FIS1 (IP:10956-1-AP, 4ug; Detection:10956-1-AP 1:10000) with HeLa cells lysate 1120 ug.

  • IF Staining of HeLa using 10956-1-AP

    IF Staining of HeLa using 10956-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FIS1 antibody (10956-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue, mouse heart tissue, SH-SY5Y cells, pig brain tissue, HeLa cells, rat heart tissue, Jurkat cells, mouse spleen tissue, rat spleen tissue, HepG2 cells, PC-12 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, Hepa1-6 cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10956-1-AP targets FIS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig, chicken, zebrafish, bovine, hamster, goat, squirrel
Immunogen FIS1 fusion protein Ag1409 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Synonyms TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
RRIDAB_2102532
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

实验方案

Product Specific Protocols
WB protocol for FIS1 antibody 10956-1-APDownload protocol
IHC protocol for FIS1 antibody 10956-1-APDownload protocol
IF protocol for FIS1 antibody 10956-1-APDownload protocol
IP protocol for FIS1 antibody 10956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Restoring metabolism of myeloid cells reverses cognitive decline in ageing.

Authors - Paras S Minhas
View Article
WB,IF

Nature

Distinct fission signatures predict mitochondrial degradation or biogenesis.

Authors - Tatjana Kleele
  • KO Validated
View Article
humanWB

Nat Immunol

RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.

Authors - Xiaqiong Wang
  • KD Validated
View Article
humanWB,IF

Cell Stem Cell

AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells.

Authors - Shanshan Pei
  • KD Validated
View Article
humanWB

Cell Metab

Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.

Authors - Jing Yuan
View Article
mouseWB,IP

Nat Metab

Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice

Authors - Wan-Ting Dong
View Article