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验证数据展示

  • IF Staining of HepG2 using CL488-10956

    IF Staining of HepG2 using CL488-10956

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FIS1 antibody (CL488-10956) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FIS1 Polyclonal antibody

FIS1 Polyclonal Antibody for IF/ICC
Cat No. CL488-10956

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Host / Isotype

Rabbit / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10956-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10956 targets FIS1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen FIS1 fusion protein Ag1409 种属同源性预测
Host / Isotype Rabbit / IgG1
Class Polyclonal
Type Antibody
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Synonyms CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
RRIDAB_2923730
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FIS1 antibody CL488-10956Download protocol
Standard Protocols
Click here to view our Standard Protocols