验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-10956 targets FIS1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat, pig |
免疫原 | FIS1 fusion protein Ag1409 种属同源性预测 |
宿主/亚型 | Rabbit / IgG1 |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) |
别名 | CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11 |
计算分子量 | 17 kDa |
观测分子量 | 17 kDa |
GenBank蛋白编号 | BC009428 |
基因名称 | FIS1 |
Gene ID (NCBI) | 51024 |
RRID | AB_2923730 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y3D6 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 FIS1 antibody CL488-10956 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |