验证数据展示

WB analysis using 10956-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 10956-1-AP
WB result of FIS1 antibody (10956-1-AP, 1:8,000) with si-Control and si-FIS1 transfected HeLa cells.
IF Staining of Hepa1-6 cells using 10956-1-AP
IF result of anti-FIS1(10956-1-AP,1:100) with Hepa1-6 cell by Dr. Steven Eugene Smith. Mitochondrion outer membrane (Green) Stain.
IF Staining of HepG2 using 10956-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10956-1-AP (FIS1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IHC staining of rat brain using 10956-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10956-1-AP (FIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis using 10956-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10956-1-AP
IP result of anti-FIS1 (IP:10956-1-AP, 4ug; Detection:10956-1-AP 1:10000) with HeLa cells lysate 1120 ug.
IF Staining of HeLa using 10956-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FIS1 antibody (10956-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

FIS1 Polyclonal antibody

FIS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10956-1-AP

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Publications(342)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Synonyms

TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size:

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概述

经过测试的应用
产品信息
发表文章 (342)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, mouse brain tissue, rat brain tissue, mouse heart tissue, SH-SY5Y cells, pig brain tissue, HeLa cells, rat heart tissue, Jurkat cells, mouse spleen tissue, rat spleen tissue, HepG2 cells, PC-12 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	rat brain tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, Hepa1-6 cells, HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:14000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 11 publications below
WB	See 315 publications below
IHC	See 12 publications below
IF	See 39 publications below
IP	See 6 publications below
CoIP	See 1 publications below

产品信息

10956-1-AP targets FIS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP
Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, duck
Immunogen	FIS1 fusion protein Ag1409 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Synonyms	TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135
Calculated Molecular Weight	17 kDa
Observed Molecular Weight	17 kDa
GenBank Accession Number	BC009428
Gene Symbol	FIS1
Gene ID (NCBI)	51024
RRID	AB_2102532
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y3D6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

实验方案

Product Specific Protocols
WB protocol for FIS1 antibody 10956-1-AP	Download protocol
IHC protocol for FIS1 antibody 10956-1-AP	Download protocol
IF protocol for FIS1 antibody 10956-1-AP	Download protocol
IP protocol for FIS1 antibody 10956-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nature Restoring metabolism of myeloid cells reverses cognitive decline in ageing. Authors - Paras S Minhas View Article
	WB,IF	Nature Distinct fission signatures predict mitochondrial degradation or biogenesis. Authors - Tatjana Kleele KO Validated View Article
human	WB	Nat Immunol RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway. Authors - Xiaqiong Wang KD Validated View Article
human	WB,IF	Cell Stem Cell AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells. Authors - Shanshan Pei KD Validated View Article
human	WB	Cell Metab Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae. Authors - Jing Yuan View Article
mouse	WB,IP	Nat Metab Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice Authors - Wan-Ting Dong View Article

验证数据展示

WB analysis using 10956-1-AP

WB analysis of HeLa cells using 10956-1-AP

IF Staining of Hepa1-6 cells using 10956-1-AP

IF Staining of HepG2 using 10956-1-AP

IHC staining of rat brain using 10956-1-AP

WB analysis using 10956-1-AP

IP experiment of HeLa using 10956-1-AP

IF Staining of HeLa using 10956-1-AP

FIS1 Polyclonal antibody

FIS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Restoring metabolism of myeloid cells reverses cognitive decline in ageing.

Distinct fission signatures predict mitochondrial degradation or biogenesis.

RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.

AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells.

Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.

Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice