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FIS1 Polyclonal antibody

FIS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10956-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig and More (6)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue, mouse heart tissue, SH-SY5Y cells, pig brain tissue, HeLa cells, rat heart tissue, Jurkat cells, mouse spleen tissue, rat spleen tissue, HepG2 cells, PC-12 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, Hepa1-6 cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10956-1-AP targets FIS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, duck
免疫原 FIS1 fusion protein Ag1409 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
别名 TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135
计算分子量 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC009428
基因名称 FIS1
Gene ID (NCBI) 51024
RRIDAB_2102532
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3D6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

实验方案

Product Specific Protocols
WB protocol for FIS1 antibody 10956-1-APDownload protocol
IHC protocol for FIS1 antibody 10956-1-APDownload protocol
IF protocol for FIS1 antibody 10956-1-APDownload protocol
IP protocol for FIS1 antibody 10956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Restoring metabolism of myeloid cells reverses cognitive decline in ageing.

Authors - Paras S Minhas
WB,IF

Nature

Distinct fission signatures predict mitochondrial degradation or biogenesis.

Authors - Tatjana Kleele
  • KO Validated
humanWB

Nat Immunol

RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.

Authors - Xiaqiong Wang
  • KD Validated
humanWB,IF

Cell Stem Cell

AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells.

Authors - Shanshan Pei
  • KD Validated
humanWB

Cell Metab

Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.

Authors - Jing Yuan
mouseWB,IP

Nat Metab

Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice

Authors - Wan-Ting Dong
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