验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse brain tissue, rat brain tissue, mouse heart tissue, SH-SY5Y cells, pig brain tissue, HeLa cells, rat heart tissue, Jurkat cells, mouse spleen tissue, rat spleen tissue, HepG2 cells, PC-12 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | rat brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, Hepa1-6 cells, HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:14000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10956-1-AP targets FIS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse, rat, pig |
文献引用反应性 | human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, duck |
免疫原 | FIS1 fusion protein Ag1409 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) |
别名 | TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135 |
计算分子量 | 17 kDa |
观测分子量 | 17 kDa |
GenBank蛋白编号 | BC009428 |
基因名称 | FIS1 |
Gene ID (NCBI) | 51024 |
RRID | AB_2102532 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y3D6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)
实验方案
Product Specific Protocols | |
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WB protocol for FIS1 antibody 10956-1-AP | Download protocol |
IHC protocol for FIS1 antibody 10956-1-AP | Download protocol |
IF protocol for FIS1 antibody 10956-1-AP | Download protocol |
IP protocol for FIS1 antibody 10956-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature Distinct fission signatures predict mitochondrial degradation or biogenesis.
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Nat Immunol RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.
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Cell Stem Cell AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells.
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Nat Metab Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice |