PYCR1-specific Polyclonal antibody

PYCR1-specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20962-1-AP

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Synonyms

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1



经过测试的应用

Positive WB detected inRaji cells, human brain tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PYCR1 Ready-To-Use IHC Kit. PYCR1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20962-1-AP targets PYCR1-specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyrroline-5-carboxylate reductase 1
Synonyms P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberNM_006907
Gene Symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_10638486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32322
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody is specific to PYCR1.

实验方案

Product Specific Protocols
WB protocol for PYCR1-specific antibody 20962-1-APDownload protocol
IHC protocol for PYCR1-specific antibody 20962-1-APDownload protocol
IP protocol for PYCR1-specific antibody 20962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Authors - Simon Schwörer
human,mouseWB

EMBO Mol Med

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy

Authors - Nneka Southwell