验证数据展示
经过测试的应用
| Positive WB detected in | human peripheral blood platelets, Jurkat cells |
| Positive IP detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60322-1-Ig targets P-selectin / CD62P in WB, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | P-selectin / CD62P fusion protein Ag4124 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | selectin P (granule membrane protein 140kDa, antigen CD62) |
| 别名 | CD62P, P-selectin, GMP140, GMP 140, CD62 antigen-like family member P |
| 计算分子量 | 830 aa, 91 kDa |
| 观测分子量 | 140 kDa, 105-110 kDa |
| GenBank蛋白编号 | BC028067 |
| 基因名称 | CD62P |
| Gene ID (NCBI) | 6403 |
| RRID | AB_2881433 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P16109 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P-selectin / CD62P antibody 60322-1-Ig | Download protocol |
| IP protocol for P-selectin / CD62P antibody 60322-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Acta Biomater Rescuing ischemic stroke by biomimetic nanovesicles through accelerated thrombolysis and sequential ischemia-reperfusion protection. | ||
Front Oncol Platelet Membranes Coated Gold Nanocages for Tumor Targeted Drug Delivery and Amplificated Low-Dose Radiotherapy. | ||
Commun Biol Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells. | ||
Front Bioeng Biotechnol Nano-Platelets as an Oxygen Regulator for Augmenting Starvation Therapy Against Hypoxic Tumor. |
