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P-selectin / CD62P Monoclonal antibody, PBS Only

P-selectin / CD62P Monoclonal Antibody for WB,IP,Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,IP,Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G2C7

Cat No : 60322-1-PBS

Print datasheet

Synonyms

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP

验证数据展示

  • WB analysis using 60322-1-Ig (same clone as 60322-1-PBS)

    WB analysis using 60322-1-Ig (same clone as 60322-1-PBS)

    Untreated and PNGase F-treated lysates of human platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood platelets using 60322-1-Ig (same clone as 60322-1-PBS)

    WB analysis of human peripheral blood platelets using 60322-1-Ig (same clone as 60322-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 60322-1-Ig (same clone as 60322-1-PBS)

    IP experiment of K-562 using 60322-1-Ig (same clone as 60322-1-PBS)

    IP result of anti-P-selectin / CD62P (IP:60322-1-Ig, 5ug; Detection:60322-1-Ig 1:600) with K-562 cells lysate 1760ug. This data was developed using the same antibody clone with 60322-1-PBS in a different storage buffer formulation.

产品信息

60322-1-PBS targets P-selectin / CD62P in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,IP,Indirect ELISA Application Description
Tested Reactivity human
Immunogen P-selectin / CD62P fusion protein Ag4124 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Synonyms CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP
Calculated Molecular Weight 830 aa, 91 kDa
Observed Molecular Weight 140 kDa, 105-110 kDa
GenBank Accession NumberBC028067
Gene Symbol SELP
Gene ID (NCBI) 6403
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16109
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.