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GLUT1 Polyclonal antibody

GLUT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Cat no : 21829-1-AP

Print datasheet

Synonyms

SLC2A1, SLC2A1,GLUT1, GLUT-1



经过测试的应用

Positive WB detected inunboiled HT-29 cells, 37°C incubated mouse colon tissue
Positive IHC detected inrat brain tissue, human lung cancer tissue, human cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

For optimal WB detection with 21829-1-AP, we recommend to avoid boiling the sample after lysis.

Planning an IHC experiment? We recommend our IHCeasy GLUT1 Ready-To-Use IHC Kit. GLUT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21829-1-AP targets GLUT1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, goat, lasiopodomys brandtii
Immunogen GLUT1 fusion protein Ag16282 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Synonyms SLC2A1, SLC2A1,GLUT1, GLUT-1
Calculated Molecular Weight 492 aa, 54 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC121804
Gene Symbol GLUT1
Gene ID (NCBI) 6513
RRIDAB_10837075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.

What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?

There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).

What is the subcellular localization of GLUT1?

Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.

What molecules can be transported by GLUT1?

The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate.

What is the tissue expression pattern of GLUT1?

GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.



实验方案

Product Specific Protocols
WB protocol for GLUT1 antibody 21829-1-APDownload protocol
IHC protocol for GLUT1 antibody 21829-1-APDownload protocol
IF protocol for GLUT1 antibody 21829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Adv Mater

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Authors - Zhuo Li
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
mouseWB,IHC

Int J Oral Sci

Transcriptional activation of glucose transporter 1 in orthodontic tooth movement-associated mechanical response.

Authors - Yu Wang
mouseIF

ACS Nano

Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer

Authors - Lingtong Meng
mouseIF

Neuron

Sympathetic nerve-enteroendocrine L cell communication modulates GLP-1 release, brain glucose utilization, and cognitive function

Authors - Wenran Ren
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma