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CoraLite® Plus 488-conjugated GLUT1 Polyclonal antibody

GLUT1 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-21829

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

SLC2A1, SLC2A1,GLUT1, GLUT-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inJurkat cells

For optimal WB detection with 21829-1-AP, we recommend to avoid boiling the sample after lysis.

Planning an IHC experiment? We recommend our IHCeasy GLUT1 Ready-To-Use IHC Kit. GLUT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21829-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21829 targets GLUT1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 GLUT1 fusion protein Ag16282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 2 (facilitated glucose transporter), member 1
别名 SLC2A1, SLC2A1,GLUT1, GLUT-1
计算分子量 492 aa, 54 kDa
观测分子量 45-55 kDa
GenBank蛋白编号BC121804
基因名称 GLUT1
Gene ID (NCBI) 6513
RRIDAB_3672737
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11166
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.

What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?

There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).

What is the subcellular localization of GLUT1?

Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.

What molecules can be transported by GLUT1?

The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate.

What is the tissue expression pattern of GLUT1?

GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.



实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GLUT1 antibody CL488-21829Download protocol
FC protocol for CL Plus 488 GLUT1 antibody CL488-21829Download protocol
Standard Protocols
Click here to view our Standard Protocols
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