验证数据展示

WB analysis of HepG2 using 17479-1-AP
WB result of YTHDF1 antibody (17479-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF1 transfected HepG2 cells.
WB analysis using 17479-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17479-1-AP (YTHDF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 17479-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17479-1-AP (YTHDF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IF Staining of mouse brain using 17479-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using YTHDF1 antibody (17479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human kidney using 17479-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17479-1-AP (YTHDF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with YTHDF1 antibody included
WB analysis using 17479-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17479-1-AP (YTHDF1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 17479-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17479-1-AP (YTHDF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with YTHDF1 antibody included
IP experiment of mouse brain using 17479-1-AP
IP result of anti-YTHDF1 (IP:17479-1-AP, 4ug; Detection:17479-1-AP 1:1000) with mouse brain tissue lysate 2640ug.
IF Staining of U2OS using 17479-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF1 antibody (17479-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO验证

YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 17479-1-AP

产品说明书

引用文献(252)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (3)

别名

YTH domain-containing family protein 1, DF1, DACA-1, C20orf21

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (252)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, HepG2 cells, A549 cells, mouse brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human kidney tissue, human hypothalamus tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	U2OS cells

Planning an IHC experiment? We recommend our IHCeasy YTHDF1 Ready-To-Use IHC Kit. YTHDF1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 81 publications below
WB	See 215 publications below
IHC	See 36 publications below
IF	See 37 publications below
IP	See 8 publications below
FC	See 1 publications below
CoIP	See 4 publications below
RIP	See 58 publications below

产品信息

17479-1-AP targets YTHDF1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, RIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, monkey, chicken, zebrafish
免疫原	YTHDF1 fusion protein Ag11509 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	YTH domain family, member 1
别名	YTH domain-containing family protein 1, DF1, DACA-1, C20orf21
计算分子量	559 aa, 61 kDa
观测分子量	60 kDa
GenBank蛋白编号	BC050284
基因名称	YTHDF1
Gene ID (NCBI)	54915
RRID	AB_2217473
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9BYJ9
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa.

实验方案

Product Specific Protocols
WB protocol for YTHDF1 antibody 17479-1-AP	Download protocol
IHC protocol for YTHDF1 antibody 17479-1-AP	Download protocol
IF protocol for YTHDF1 antibody 17479-1-AP	Download protocol
IP protocol for YTHDF1 antibody 17479-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Nature m6A enhances the phase separation potential of mRNA. Authors - Ryan J Ries View Article
mouse	WB	Nature m6A RNA methylation regulates the fate of endogenous retroviruses. Authors - Tomasz Chelmicki View Article
mouse	WB,IF	Nature m6A facilitates hippocampus-dependent learning and memory through YTHDF1. Authors - Hailing Shi KO Validated View Article
human	WB	Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Authors - Peiguo Yang View Article
human	WB	Cell A Unified Model for the Function of YTHDF Proteins in Regulating m6A-Modified mRNA. Authors - Sara Zaccara KD Validated View Article
mouse	WB	Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Authors - Deniz Simsek View Article

验证数据展示

WB analysis of HepG2 using 17479-1-AP

WB analysis using 17479-1-AP

WB analysis using 17479-1-AP

IF Staining of mouse brain using 17479-1-AP

IHC staining of human kidney using 17479-1-AP

WB analysis using 17479-1-AP

IHC staining of human kidney using 17479-1-AP

IP experiment of mouse brain using 17479-1-AP

IF Staining of U2OS using 17479-1-AP

YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

m6A enhances the phase separation potential of mRNA.

m6A RNA methylation regulates the fate of endogenous retroviruses.

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

A Unified Model for the Function of YTHDF Proteins in Regulating m6A-Modified mRNA.

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.