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验证数据展示

  • IF Staining of mouse brain using CL594-17479

    IF Staining of mouse brain using CL594-17479

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 YTHDF1 antibody (CL594-17479) at dilution of 1:200.

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  • KD/KO验证

CoraLite®594-conjugated YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for IF-P
Cat No. CL594-17479

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P

别名

C20orf21, DACA 1, FLJ20391, YTH domain family protein 1, YTH domain family, member 1, YTHDF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy YTHDF1 Ready-To-Use IHC Kit. YTHDF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17479-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17479 targets YTHDF1 in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原 YTHDF1 fusion protein Ag11509 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YTH domain family, member 1
别名 C20orf21, DACA 1, FLJ20391, YTH domain family protein 1, YTH domain family, member 1, YTHDF1
计算分子量 559 aa, 61 kDa
观测分子量 60 kDa
GenBank蛋白编号BC050284
基因名称 YTHDF1
Gene ID (NCBI) 54915
RRIDAB_2919841
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYJ9
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 YTHDF1 antibody CL594-17479Download protocol
Standard Protocols
Click here to view our Standard Protocols